5KX5
Crystal structure of tubulin-stathmin-TTL-Compound 11 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.790, 153.900, 185.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.470 - 2.500 |
R-factor | 0.1996 |
Rwork | 0.197 |
R-free | 0.25140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i55 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data scaling software | SCALEPACK |
Refinement software | BUSTER-TNT (2.11.6) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.470 | 100.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.790 | 2.500 |
Rmerge | 0.152 | 0.045 | |
Number of reflections | 101681 | ||
<I/σ(I)> | 3.6 | ||
Completeness [%] | 97.8 | 99.6 | 78.4 |
Redundancy | 5.9 | 6.3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | precipitant solution: 3% PEG 4K, 4-6% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole pH 6.7 |