5KSW
DHODB-I74D mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, C) | Dihydroorotate dehydrogenase | polymer | 306 | 32344.5 | 2 | UniProt (A0A089ZD72) Pfam (PF01180) UniProt (by SIFTS) (Q9CFW8) | Lactococcus lactis | DHOdehase |
| 2 | B, C (B, D) | Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit | polymer | 262 | 28862.6 | 2 | UniProt (A0A0V8EGX5) Pfam (PF00175) Pfam (PF10418) UniProt (by SIFTS) (Q9CFW7) | Lactococcus lactis subsp. lactis | Dihydroorotate oxidase B,electron transfer subunit |
| 3 | E, M (A, C) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) | |||
| 4 | F, J (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 5 | G, N (A, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 6 | H, K (B, D) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 2 | Chemie (FAD) | |||
| 7 | I, L (B, D) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 8 | O, P, Q, R (A, B, D, C) | water | water | 18.0 | 389 | Chemie (HOH) |
Sequence modifications
A, C: 5 - 310 (UniProt: A0A089ZD72)
| PDB | External Database | Details |
|---|---|---|
| Ala 67 | Ser 67 | conflict |
| Asp 74 | Ile 74 | engineered mutation |
| Val 128 | Ala 128 | conflict |
| Ala 187 | His 187 | conflict |
| Gly 189 | Cys 189 | conflict |
| Asn 308 | Lys 308 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 122414.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3030.5 | |
| All* | Total formula weight | 125444.7 |
