5KSW
DHODB-I74D mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004152 | molecular_function | dihydroorotate dehydrogenase activity |
| A | 0004589 | molecular_function | dihydroorotate dehydrogenase (NAD+) activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| A | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| A | 0006222 | biological_process | UMP biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016627 | molecular_function | oxidoreductase activity, acting on the CH-CH group of donors |
| A | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| B | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| B | 0009055 | molecular_function | electron transfer activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0051536 | molecular_function | iron-sulfur cluster binding |
| B | 0051537 | molecular_function | 2 iron, 2 sulfur cluster binding |
| C | 0004152 | molecular_function | dihydroorotate dehydrogenase activity |
| C | 0004589 | molecular_function | dihydroorotate dehydrogenase (NAD+) activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| C | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| C | 0006222 | biological_process | UMP biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016627 | molecular_function | oxidoreductase activity, acting on the CH-CH group of donors |
| C | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| D | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| D | 0009055 | molecular_function | electron transfer activity |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| D | 0051536 | molecular_function | iron-sulfur cluster binding |
| D | 0051537 | molecular_function | 2 iron, 2 sulfur cluster binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | binding site for residue FMN A 401 |
| Chain | Residue |
| A | ALA23 |
| A | ASN132 |
| A | LYS170 |
| A | ILE196 |
| A | ASN197 |
| A | SER221 |
| A | GLY222 |
| A | MET247 |
| A | GLY248 |
| A | GLY249 |
| A | GLY270 |
| A | SER24 |
| A | THR271 |
| A | HOH533 |
| A | HOH536 |
| A | HOH554 |
| A | GLY25 |
| A | CYS26 |
| A | LYS48 |
| A | ALA49 |
| A | ASN72 |
| A | LEU76 |
| A | ASN104 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue CL A 402 |
| Chain | Residue |
| A | THR51 |
| A | LEU52 |
| A | HIS53 |
| A | HOH534 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 403 |
| Chain | Residue |
| A | ARG203 |
| A | LYS226 |
| A | HOH526 |
| A | HOH541 |
| site_id | AC4 |
| Number of Residues | 22 |
| Details | binding site for residue FAD B 301 |
| Chain | Residue |
| B | ARG53 |
| B | PRO54 |
| B | ILE55 |
| B | SER56 |
| B | LEU70 |
| B | TYR71 |
| B | ARG72 |
| B | SER78 |
| B | GLY79 |
| B | THR80 |
| B | ILE120 |
| B | GLU221 |
| B | SER222 |
| B | ARG223 |
| B | MET224 |
| B | HOH405 |
| B | HOH409 |
| B | HOH430 |
| B | HOH431 |
| B | HOH440 |
| B | HOH446 |
| B | HOH447 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | binding site for residue FES B 302 |
| Chain | Residue |
| B | ALA225 |
| B | CYS226 |
| B | GLY227 |
| B | GLY229 |
| B | CYS231 |
| B | TYR232 |
| B | CYS234 |
| B | CYS249 |
| site_id | AC6 |
| Number of Residues | 1 |
| Details | binding site for residue CL B 303 |
| Chain | Residue |
| B | VAL63 |
| site_id | AC7 |
| Number of Residues | 20 |
| Details | binding site for residue FAD D 301 |
| Chain | Residue |
| D | ARG53 |
| D | PRO54 |
| D | ILE55 |
| D | SER56 |
| D | LEU70 |
| D | TYR71 |
| D | ARG72 |
| D | SER78 |
| D | GLY79 |
| D | THR80 |
| D | ILE120 |
| D | GLU221 |
| D | SER222 |
| D | ARG223 |
| D | MET224 |
| D | HOH404 |
| D | HOH422 |
| D | HOH431 |
| D | HOH444 |
| D | HOH453 |
| site_id | AC8 |
| Number of Residues | 22 |
| Details | binding site for residue FMN C 401 |
| Chain | Residue |
| C | GLY249 |
| C | GLY270 |
| C | THR271 |
| C | HOH502 |
| C | HOH511 |
| C | ALA23 |
| C | SER24 |
| C | GLY25 |
| C | LYS48 |
| C | ALA49 |
| C | ASN72 |
| C | LEU76 |
| C | ASN104 |
| C | ASN132 |
| C | LYS170 |
| C | ILE196 |
| C | ASN197 |
| C | THR198 |
| C | SER221 |
| C | GLY222 |
| C | MET247 |
| C | GLY248 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 402 |
| Chain | Residue |
| C | ALA67 |
| C | ARG203 |
| C | LYS226 |
| C | HOH521 |
| C | HOH533 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Nucleophile"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 30 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 202 |
| Details | Domain: {"description":"FAD-binding FR-type","evidences":[{"source":"HAMAP-Rule","id":"MF_01211","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 20 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01211","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
