5KS8

Crystal structure of two-subunit pyruvate carboxylase from Methylobacillus flagellatus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Pyruvate carboxylase subunit alpha	polymer	405	45053.4	2	UniProt (Q1H158) Pfam (PF00289) Pfam (PF02786) Pfam (PF02785)	Methylobacillus flagellatus
2	C, D, E, F (C, D, E, F)	Pyruvate carboxylase subunit beta	polymer	617	67066.0	4	UniProt (Q1H157) Pfam (PF00682) Pfam (PF02436) Pfam (PF00364)	Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
3	G (C)	5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL	non-polymer		228.3	1	Chemie (BTI)
4	H, J (C, D)	PYRUVIC ACID	non-polymer		88.1	2	Chemie (PYR)
5	I, K, L (C, D, F)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)

Sequence modifications

A, B: 1 - 130 (UniProt: Q1H158)
A, B: 202 - 472 (UniProt: Q1H158)

PDB	External Database	Details
Gly 131	-	linker
Ser 132	-	linker
Ser 200	-	linker
Gly 201	-	linker

C, D, E, F: 1 - 617 (UniProt: Q1H157)

PDB	External Database	Details
Ala 419	Lys 419	conflict
Ala 421	Glu 421	conflict
Ala 422	Glu 422	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	358370.9
Non-Polymers*	Number of molecules	6
	Total formula weight	569.2
All*	Total formula weight	358940.1

*Water molecules are not included.