5KOV

Crystal structure of the human astrovirus 2 capsid protein spike in complex with a single chain variable fragment of an astrovirus neutralizing antibody at 3.24-A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, E, H, I... (A, B, H, G, N...)	Capsid polyprotein VP90	polymer	228	25976.6	8	UniProt (Q82446) Pfam (PF12226)	Human astrovirus-2 (HAstV-2)
2	B, C, F, G, J... (C, E, K, I, Q...)	PL-2 scFv chain	polymer	251	26732.5	8		Mus musculus
3	Q, R, S, T (C, E, K, I)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, B, H, G, N, M, S, T: 429 - 644 (UniProt: Q82446)

PDB	External Database	Details
Met 427	-	initiating methionine
Gly 428	-	expression tag
Ala 645	-	expression tag
Ala 646	-	expression tag
Ala 647	-	expression tag
Glu 648	-	expression tag
Leu 649	-	expression tag
Ala 650	-	expression tag
Leu 651	-	expression tag
Val 652	-	expression tag
Pro 653	-	expression tag
Arg 654	-	expression tag

C, E, K, I, Q, O, W, U: -1 - 1000 (PDB: 5KOV)
C, E, K, I, Q, O, W, U: 1001 - 1112 (PDB: 5KOV)

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	421672.6
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	422557.5

*Water molecules are not included.