5KNL

Crystal structure of S. pombe ubiquitin E1 (Uba1) in complex with Ubc15 and ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Ubiquitin-activating enzyme E1 1	polymer	1001	111764.0	2	UniProt (O94609) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)	Poly(A)+ RNA transport protein 3
2	B, E	Ubiquitin	polymer	96	10904.2	2	UniProt (A0A081CFF0) Pfam (PF00240) UniProt (by SIFTS) (A0A081CEG8) In PDB	Pseudozyma antarctica (Yeast)
3	C, F	Ubiquitin-conjugating enzyme E2 15	polymer	175	20199.9	2	UniProt (Q9Y818) Pfam (PF00179) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)	E2 ubiquitin-conjugating enzyme 15,Ubiquitin carrier protein 15,Ubiquitin-protein ligase 15
4	A, B, D, F	SULFATE ION	non-polymer		96.1	9	Chemie (SO4)
5		water	water		18.0	94	Chemie (HOH)

Sequence modifications

A, D: 13 - 1012 (UniProt: O94609)

PDB	External Database	Details
Ser 12	-	expression tag

B, E: 1 - 76 (UniProt: A0A081CFF0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 6	Lys 35	engineered mutation
Arg 11	Lys 40	engineered mutation
Arg 27	Lys 56	engineered mutation
Arg 29	Lys 58	engineered mutation
Arg 33	Lys 62	engineered mutation
Arg 48	Lys 77	engineered mutation
Ala 57	Ser 86	engineered mutation
Arg 63	Lys 92	engineered mutation

C, F: 1 - 167 (UniProt: Q9Y818)

PDB	External Database	Details
Leu 168	-	expression tag
Glu 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	285736.4
Non-Polymers*	Number of molecules	9
	Total formula weight	864.6
All*	Total formula weight	286600.9

*Water molecules are not included.