5KNL

Crystal structure of S. pombe ubiquitin E1 (Uba1) in complex with Ubc15 and ubiquitin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0004839	molecular_function	ubiquitin activating enzyme activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0006974	biological_process	DNA damage response
A	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
A	0016567	biological_process	protein ubiquitination
A	0016874	molecular_function	ligase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0036211	biological_process	protein modification process
A	0046872	molecular_function	metal ion binding
C	0000166	molecular_function	nucleotide binding
C	0000209	biological_process	protein polyubiquitination
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0016567	biological_process	protein ubiquitination
C	0016740	molecular_function	transferase activity
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0061631	molecular_function	ubiquitin conjugating enzyme activity
C	0061650	molecular_function	ubiquitin-like protein conjugating enzyme activity
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0004839	molecular_function	ubiquitin activating enzyme activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0006974	biological_process	DNA damage response
D	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
D	0016567	biological_process	protein ubiquitination
D	0016874	molecular_function	ligase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0036211	biological_process	protein modification process
D	0046872	molecular_function	metal ion binding
F	0000166	molecular_function	nucleotide binding
F	0000209	biological_process	protein polyubiquitination
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0016567	biological_process	protein ubiquitination
F	0016740	molecular_function	transferase activity
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0061631	molecular_function	ubiquitin conjugating enzyme activity
F	0061650	molecular_function	ubiquitin-like protein conjugating enzyme activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue SO4 A 1101

Chain	Residue
A	HIS29
A	GLU30

---

site_id	AC2
Number of Residues	5
Details	binding site for residue SO4 A 1102

Chain	Residue
A	PHE590
A	ARG601
A	HIS604
C	ASP100
C	ARG110

---

site_id	AC3
Number of Residues	5
Details	binding site for residue SO4 A 1103

Chain	Residue
A	GLU603
A	LYS843
C	LYS101
A	ARG601
A	ILE602

---

site_id	AC4
Number of Residues	3
Details	binding site for residue SO4 A 1104

Chain	Residue
A	ARG22
A	ASN471
A	ARG474

---

site_id	AC5
Number of Residues	3
Details	binding site for residue SO4 B 101

Chain	Residue
A	ARG201
B	ARG29
B	ARG33

---

site_id	AC6
Number of Residues	3
Details	binding site for residue SO4 D 1101

Chain	Residue
D	GLY28
D	HIS29
D	GLU30

---

site_id	AC7
Number of Residues	2
Details	binding site for residue SO4 D 1102

Chain	Residue
D	ARG610
D	LYS850

---

site_id	AC8
Number of Residues	2
Details	binding site for residue SO4 D 1103

Chain	Residue
D	ARG22
D	ARG474

---

site_id	AC9
Number of Residues	5
Details	binding site for residue SO4 F 201

Chain	Residue
D	PHE590
D	ARG601
D	HIS604
F	ASP99
F	ARG110

---

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. WHPNVhpn.GeVCIsiL

Chain	Residue	Details
C	TRP79-LEU94

---

site_id	PS00865
Number of Residues	9
Details	UBIQUITIN_ACTIVAT_2 Ubiquitin-activating enzyme active site. PICTLKnFP

Chain	Residue	Details
A	PRO591-PRO599

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU10132","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23416107","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4II2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4II3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P22515","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23416107","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4II2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P22515","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P22515","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	160
Details	Domain: {"description":"UBC core","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10133","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---