5KL4

Wilms Tumor Protein (WT1) ZnF2-4 Q369H in complex with formylated DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Wilms tumor protein	polymer	93	11292.9	2	UniProt (P19544) Pfam (PF00096) In PDB	Homo sapiens (Human)	WT33
2	B, E	DNA (5'-D(*AP*GP*CP*GP*TP*GP*GP*GP*(5FC)P*GP*T)-3')	polymer	11	3458.2	2		synthetic construct
3	C, F	DNA (5'-D(*TP*AP*(5CM)P*GP*CP*CP*CP*AP*CP*GP*C)-3')	polymer	11	3293.2	2		synthetic construct
4	A, D	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
5	A, D	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	A, D	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	F	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
8		water	water		18.0	175	Chemie (HOH)

Sequence modifications

A, D: 350 - 437 (UniProt: P19544)

PDB	External Database	Details
Gly 345	-	expression tag
Pro 346	-	expression tag
Leu 347	-	expression tag
Gly 348	-	expression tag
Ser 349	-	expression tag
His 369	Gln 352	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	36088.6
Non-Polymers*	Number of molecules	11
	Total formula weight	619.7
All*	Total formula weight	36708.4

*Water molecules are not included.