5KK7
Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase T688A mutant and Thio-disaccharide substrate analog complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase | polymer | 455 | 52269.4 | 2 | UniProt (Q9UKM7) Pfam (PF01532) | Homo sapiens (Human) | ER alpha-1,2-mannosidase,ER mannosidase 1,ERMan1,Man9GlcNAc2-specific-processing alpha-mannosidase,Mannosidase alpha class 1B member 1 |
| 2 | C, D (C, D) | alpha-D-mannopyranose-(1-2)-methyl 2-thio-alpha-D-mannopyranoside | branched | 372.4 | 2 | In PDB BIRD (PRD_900033) GlyTouCan (G32183TR) | methyl 2-S-alpha-D-mannopyranosyl-2-thio-alpha-D-mannopyranoside | ||
| 3 | E, N (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | F, G, O (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 5 | H, M (A) | 3-PYRIDINIUM-1-YLPROPANE-1-SULFONATE | non-polymer | 201.2 | 2 | Chemie (1PS) | |||
| 6 | I, J, P, S (A, B) | 1,4-BUTANEDIOL | non-polymer | 90.1 | 4 | Chemie (BU1) | |||
| 7 | K, L, Q, R, T (A, B) | ACETATE ION | non-polymer | 59.0 | 5 | Chemie (ACT) | |||
| 8 | U, V (A, B) | water | water | 18.0 | 1189 | Chemie (HOH) |
Sequence modifications
A, B: 245 - 699 (UniProt: Q9UKM7)
| PDB | External Database | Details |
|---|---|---|
| Ala 688 | Thr 688 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 104538.7 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 744.8 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 1426.5 | |
| All* | Total formula weight | 106710.0 |
