5KCM

Crystal structure of iron-sulfur cluster containing photolyase PhrB mutant I51W

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	(6-4) photolyase	polymer	518	59296.9	2	UniProt (A9CH39) Pfam (PF04244)	Agrobacterium fabrum (strain C58 / ATCC 33970)	(6-4)DNA photolyase,DNA photolyase PhrB,Photoreactivating enzyme PhrB
2	C, F (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	D, G (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
4	E, H, I, J (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	K, L (A, B)	water	water		18.0	619	Chemie (HOH)

Sequence modifications

A, B: 1 - 507 (UniProt: A9CH39)

PDB	External Database	Details
Trp 51	Ile 51	engineered mutation
Ala 508	-	expression tag
Ala 509	-	expression tag
Ala 510	-	expression tag
Leu 511	-	expression tag
Glu 512	-	expression tag
His 513	-	expression tag
His 514	-	expression tag
His 515	-	expression tag
His 516	-	expression tag
His 517	-	expression tag
His 518	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	118593.8
Non-Polymers*	Number of molecules	8
	Total formula weight	2642.8
All*	Total formula weight	121236.6

*Water molecules are not included.