5KBN

The crystal structure of fluoride channel Fluc Ec2 F80I Mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Putative fluoride ion transporter CrcB	polymer	147	15808.8	2	UniProt (Q6J5N4) Pfam (PF02537) In PDB	Escherichia coli
2	C, D	monobody	polymer	97	10375.5	2		Homo sapiens (Human)
3	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	A, B, C	DECYL-BETA-D-MALTOPYRANOSIDE	non-polymer		482.6	5	Chemie (DMU)
5	A, B	FLUORIDE ION	non-polymer		19.0	2	Chemie (F)
6		water	water		18.0	33	Chemie (HOH)

Sequence modifications

A, B: 1 - 126 (UniProt: Q6J5N4)

PDB	External Database	Details
Lys 25	Arg 25	engineered mutation
Ile 80	Phe 80	engineered mutation
Thr 127	-	expression tag
Arg 128	-	expression tag
Gly 129	-	expression tag
Lys 130	-	expression tag
Ala 131	-	expression tag
Ala 132	-	expression tag
Ser 133	-	expression tag
Leu 134	-	expression tag
Val 135	-	expression tag
Pro 136	-	expression tag
Arg 137	-	expression tag
Gly 138	-	expression tag
Ser 139	-	expression tag
Gly 140	-	expression tag
Gly 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52368.6
Non-Polymers*	Number of molecules	8
	Total formula weight	2473.8
All*	Total formula weight	54842.4

*Water molecules are not included.