5KBN
The crystal structure of fluoride channel Fluc Ec2 F80I Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99986 |
Spacegroup name | P 41 |
Unit cell lengths | 87.655, 87.655, 143.625 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.890 - 2.480 |
R-factor | 0.2197 |
Rwork | 0.219 |
R-free | 0.23890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a43 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.192 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.200 | 39.200 | 2.580 |
High resolution limit [Å] | 2.480 | 8.940 | 2.480 |
Rmerge | 0.085 | 0.027 | 1.419 |
Number of reflections | 38347 | ||
<I/σ(I)> | 20.4 | ||
Completeness [%] | 99.9 | 96.9 | 100 |
Redundancy | 15 | 14.9 | 15.2 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 295 | 31% PEG 600, 100 mM ADA, 10 mM HEPES, 100 mM NaF, 50 mM LiNO3 |