5K98

Structure of HipA-HipB-O2-O3 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Serine/threonine-protein kinase HipA	polymer	448	50423.9	2	UniProt (P23874) Pfam (PF13657) Pfam (PF07804)	Escherichia coli (strain K12)	Ser/Thr-protein kinase HipA,Toxin HipA
2	B, E (B, P)	Antitoxin HipB	polymer	91	10337.6	2	UniProt (M9IJX7) Pfam (PF01381) UniProt (by SIFTS) (P23873)	Escherichia coli MP020980.2
3	C (T)	DNA (5'-D(*TP*CP*CP*CP*TP*AP*TP*CP*CP*CP*CP*TP*TP*AP*AP*GP*GP*GP*GP*AP*TP*AP*G)-3')	polymer	23	7016.5	1		synthetic construct
4	F (E)	DNA (5'-D(*CP*TP*AP*TP*CP*CP*CP*CP*TP*TP*AP*AP*GP*GP*GP*GP*AP*TP*AP*GP*GP*GP*A)-3')	polymer	23	7105.6	1		synthetic construct

Sequence modifications

A, D: 2 - 440 (UniProt: P23874)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag
Arg 1	-	expression tag
Gln 309	Asp 309	engineered mutation

B, P: 1 - 88 (UniProt: M9IJX7)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	135645.1
All*	Total formula weight	135645.1

*Water molecules are not included.