Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

5K1X

Catalytic domain of polyspecific pyrrolysyl-tRNA synthetase mutant Y306A/N346A/C348A/Y384F in complex with AMPPNP

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
Pyrrolysine--tRNA ligasepolymer27431661.31UniProt (Q8PWY1)
Pfam (PF01409)
Methanosarcina mazeiPyrrolysine--tRNA(Pyl) ligase,Pyrrolysyl-tRNA synthetase,PylRS
2B
(A)
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTERnon-polymer506.21Chemie (ANP)
3C, D
(A)
MAGNESIUM IONnon-polymer24.32Chemie (MG)
4E
(A)
1,2-ETHANEDIOLnon-polymer62.11Chemie (EDO)
5F
(A)
DI(HYDROXYETHYL)ETHERnon-polymer106.11Chemie (PEG)
6G
(A)
CHLORIDE IONnon-polymer35.51Chemie (CL)
7H
(A)
waterwater18.0137Chemie (HOH)
Sequence modifications
A: 188 - 454 (UniProt: Q8PWY1)
PDBExternal DatabaseDetails
Met 187-initiating methionine
Ala 306Tyr 306engineered mutation
Ala 346Asn 346engineered mutation
Ala 348Cys 348engineered mutation
Phe 384Tyr 384engineered mutation
His 455-expression tag
His 456-expression tag
His 457-expression tag
His 458-expression tag
His 459-expression tag
His 460-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains1
Total formula weight31661.3
Non-Polymers*Number of molecules6
Total formula weight758.4
All*Total formula weight32419.8
*Water molecules are not included.

239492

PDB entries from 2025-07-30

PDB statisticsPDBj update infoContact PDBjnumon