5K1X
Catalytic domain of polyspecific pyrrolysyl-tRNA synthetase mutant Y306A/N346A/C348A/Y384F in complex with AMPPNP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Pyrrolysine--tRNA ligase | polymer | 274 | 31661.3 | 1 | UniProt (Q8PWY1) Pfam (PF01409) In PDB | Methanosarcina mazei | Pyrrolysine--tRNA(Pyl) ligase,Pyrrolysyl-tRNA synthetase,PylRS |
2 | A | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
3 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
4 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
5 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
6 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
7 | water | water | 18.0 | 137 | Chemie (HOH) |
Sequence modifications
A: 188 - 454 (UniProt: Q8PWY1)
PDB | External Database | Details |
---|---|---|
Met 187 | - | initiating methionine |
Ala 306 | Tyr 306 | engineered mutation |
Ala 346 | Asn 346 | engineered mutation |
Ala 348 | Cys 348 | engineered mutation |
Phe 384 | Tyr 384 | engineered mutation |
His 455 | - | expression tag |
His 456 | - | expression tag |
His 457 | - | expression tag |
His 458 | - | expression tag |
His 459 | - | expression tag |
His 460 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 31661.3 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 758.4 | |
All* | Total formula weight | 32419.8 |