5JYB
Crystal structure of 3 mutant of Ba3275 (S116A, E243A, H313A), the member of S66 family of serine peptidases
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | MccC family protein | polymer | 346 | 39442.6 | 2 | UniProt (Q734X3) Pfam (PF02016) Pfam (PF17676) | Bacillus cereus (strain ATCC 10987 / NRS 248) | |
| 2 | C, I, J (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 3 | D, K (A, B) | HEXAETHYLENE GLYCOL | non-polymer | 282.3 | 2 | Chemie (P6G) | |||
| 4 | E, L (A, B) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) | |||
| 5 | F (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 6 | G, O (A, B) | 2-BUTANOL | non-polymer | 74.1 | 2 | Chemie (SBT) | |||
| 7 | H, P (A, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 8 | M, N (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 9 | Q, R (A, B) | water | water | 18.0 | 666 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 343 (UniProt: Q734X3)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 116 | Ser 116 | engineered mutation |
| Ala 243 | Glu 243 | engineered mutation |
| Ala 313 | His 313 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 78885.2 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 2024.3 | |
| All* | Total formula weight | 80909.5 |
