5JYA

Structures of Streptococcus agalactiae GBS GAPDH in different enzymatic states

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glyceraldehyde-3-phosphate dehydrogenase	polymer	356	38198.8	4	UniProt (Q9ALW2) Pfam (PF00044) Pfam (PF02800)	Streptococcus agalactiae
2	E, G, I, K (A, B, C, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	F, H, J, L (A, B, C, D)	GLYCERALDEHYDE-3-PHOSPHATE	non-polymer		170.1	4	Chemie (G3H)
4	M, N, O, P (A, B, C, D)	water	water		18.0	161	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 336 (UniProt: Q9ALW2)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 152	Cys 152	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	152795.2
Non-Polymers*	Number of molecules	8
	Total formula weight	3333.9
All*	Total formula weight	156129.1

*Water molecules are not included.