5JSR

New Mechanistic Insight from Substrate and Product Bound Structures of the Metal-dependent Dimethylsulfoniopropionate Lyase DddQ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Dimethlysulfonioproprionate lyase DddQ	polymer	201	22395.0	2	UniProt (D0CY60) Pfam (PF16867)	Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157)	DMSP lyase
2	C, I (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
3	D, J (A, B)	ACRYLIC ACID	non-polymer		72.1	2	Chemie (AKR)
4	E, K (A, B)	BROMIDE ION	non-polymer		79.9	2	Chemie (BR)
5	F, G, H, L, M (A, B)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
6	N, O (A, B)	water	water		18.0	179	Chemie (HOH)

Sequence modifications

A, B: 1 - 192 (UniProt: D0CY60)

PDB	External Database	Details
Met 0	-	initiating methionine
Leu 193	-	expression tag
Glu 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	44790.0
Non-Polymers*	Number of molecules	11
	Total formula weight	592.9
All*	Total formula weight	45382.9

*Water molecules are not included.