5JQH
Structure of beta2 adrenoceptor bound to carazolol and inactive-state stabilizing nanobody, Nb60
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Endolysin,Beta-2 adrenergic receptor | polymer | 471 | 53699.3 | 2 | UniProt (P00720) UniProt (P07550) Pfam (PF00959) Pfam (PF00001) | Enterobacteria phage T4 | Lysis protein,Lysozyme,Muramidase,Beta-2 adrenoreceptor,Beta-2 adrenoceptor |
| 2 | C, D (D, C) | Nanobody60, Nb60 | polymer | 125 | 13531.0 | 2 | Lama glama | ||
| 3 | E, G (A, B) | (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol | non-polymer | 298.4 | 2 | Chemie (CAU) | |||
| 4 | F, H (A, B) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) |
Sequence modifications
A, B: 867 - 1026 (UniProt: P00720)
A, B: 1030 - 1348 (UniProt: P07550)
| PDB | External Database | Details |
|---|---|---|
| Asp 852 | - | expression tag |
| Tyr 853 | - | expression tag |
| Lys 854 | - | expression tag |
| Asp 855 | - | expression tag |
| Asp 856 | - | expression tag |
| Asp 857 | - | expression tag |
| Asp 858 | - | expression tag |
| Ala 859 | - | expression tag |
| Glu 860 | - | expression tag |
| Asn 861 | - | expression tag |
| Leu 862 | - | expression tag |
| Tyr 863 | - | expression tag |
| Phe 864 | - | expression tag |
| Gln 865 | - | expression tag |
| Gly 866 | - | expression tag |
| Gly 877 | Arg 12 | conflict |
| Thr 919 | Cys 54 | engineered mutation |
| Ala 962 | Cys 97 | engineered mutation |
| Arg 1002 | Ile 137 | conflict |
| Ala 1027 | - | linker |
| Ala 1028 | - | linker |
| Asp 1029 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Thr 1096 | Met 96 | engineered mutation |
| Thr 1098 | Met 98 | engineered mutation |
| Glu 1187 | Asn 187 | engineered mutation |
| - | Lys 235 | deletion |
| - | Ser 236 | deletion |
| - | Glu 237 | deletion |
| - | Gly 238 | deletion |
| - | Arg 239 | deletion |
| - | Phe 240 | deletion |
| - | His 241 | deletion |
| - | Val 242 | deletion |
| - | Gln 243 | deletion |
| - | Asn 244 | deletion |
| - | Leu 245 | deletion |
| - | Ser 246 | deletion |
| - | Gln 247 | deletion |
| - | Val 248 | deletion |
| - | Glu 249 | deletion |
| - | Gln 250 | deletion |
| - | Asp 251 | deletion |
| - | Gly 252 | deletion |
| - | Arg 253 | deletion |
| - | Thr 254 | deletion |
| - | Gly 255 | deletion |
| - | His 256 | deletion |
| - | Gly 257 | deletion |
| - | Leu 258 | deletion |
| - | Arg 259 | deletion |
| - | Arg 260 | deletion |
| Ile 1261 | Ser 261 | conflict |
| Asp 1262 | Ser 262 | conflict |
| Ala 1265 | Cys 265 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 134460.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1370.1 | |
| All* | Total formula weight | 135830.7 |
