5JNE

E2-SUMO-Siz1 E3-SUMO-PCNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	E3 SUMO-protein ligase SIZ1,Ubiquitin-like protein SMT3	polymer	367	42195.4	2	UniProt (Q04195) UniProt (Q12306) Pfam (PF14324) Pfam (PF02891) Pfam (PF00240)	Saccharomyces cerevisiae (Baker's yeast)	SAP and Miz-finger domain-containing protein 1,Ubiquitin-like protein ligase 1
2	B, F (B, F)	SUMO-conjugating enzyme UBC9	polymer	160	18288.8	2	UniProt (P50623) Pfam (PF00179)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Ubiquitin carrier protein 9,Ubiquitin-conjugating enzyme E2-18 kDa,Ubiquitin-protein ligase
3	C, G (C, G)	Ubiquitin-like protein SMT3	polymer	84	9720.0	2	UniProt (Q12306) Pfam (PF00240)	Saccharomyces cerevisiae (Baker's yeast)
4	D, H (D, H)	Proliferating cell nuclear antigen	polymer	258	28815.7	2	UniProt (P15873) Pfam (PF00705) Pfam (PF02747)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PCNA
5	I, Q (A, E)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	J, K, L, N, O... (A, D, E, H)	GLYCEROL	non-polymer		92.1	10	Chemie (GOL)
7	M, T (B, F)	ethane-1,2-dithiol	non-polymer		94.2	2	Chemie (6LN)
8	AA, BA, CA, DA, W... (E, F, G, H, A...)	water	water		18.0	271	Chemie (HOH)

Sequence modifications

A, E: 167 - 445 (UniProt: Q04195)

PDB	External Database	Details
Ser 165	-	expression tag
Leu 166	-	expression tag
Asp 361	Cys 361	engineered mutation
Asp 446	-	linker
Asp 447	-	linker
Asp 448	-	linker
Asp 449	-	linker
Lys 450	-	linker
Leu 451	-	linker
Arg 452	-	linker

A, E: 453 - 531 (UniProt: Q12306)
B, F: 1 - 157 (UniProt: P50623)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 5	Cys 5	engineered mutation
Lys 129	Ala 129	engineered mutation
Arg 153	Lys 153	engineered mutation

C, G: 19 - 98 (UniProt: Q12306)

PDB	External Database	Details
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Met 18	-	expression tag
Arg 19	Lys 19	engineered mutation

D, H: 1 - 258 (UniProt: P15873)

PDB	External Database	Details
Asp 77	Lys 77	engineered mutation
Glu 81	Cys 81	engineered mutation
Asp 110	Arg 110	engineered mutation
Gly 127	Lys 127	engineered mutation
Cys 164	Lys 164	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	198039.5
Non-Polymers*	Number of molecules	14
	Total formula weight	1240.2
All*	Total formula weight	199279.7

*Water molecules are not included.