5JNE
E2-SUMO-Siz1 E3-SUMO-PCNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.421, 205.882, 142.501 |
| Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
| Resolution | 48.385 - 2.850 |
| R-factor | 0.2135 |
| Rwork | 0.211 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3id2 1plq 2eke |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.411 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.385 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.107 | 0.556 |
| Number of reflections | 61981 | |
| <I/σ(I)> | 9.1 | 1.82 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M Tris-HCl (pH 8.5), 5% PEG 10,000, 0.2 M NaCl, 10% glycerol, 3% dioxane |
