5JI0

PPARgamma-RXRalpha(S427F) heterodimer in complex with SRC-1, rosiglitazone, and 9-cis-retanoic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Retinoic acid receptor RXR-alpha	polymer	242	27104.4	1	UniProt (P19793) Pfam (PF00104) In PDB	Homo sapiens (Human)	Nuclear receptor subfamily 2 group B member 1,Retinoid X receptor alpha
2	D	Peroxisome proliferator-activated receptor gamma	polymer	273	31151.2	1	UniProt (P37231) Pfam (PF00104) In PDB	Homo sapiens (Human)	PPAR-gamma,Nuclear receptor subfamily 1 group C member 3
3	E, F	Nuclear receptor coactivator 1	polymer	27	2830.2	2	UniProt (Q15788) Pfam (PF08832) In PDB	Homo sapiens	NCoA-1,Class E basic helix-loop-helix protein 74,bHLHe74,Protein Hin-2,RIP160,Renal carcinoma antigen NY-REN-52,Steroid receptor coactivator 1,SRC-1
4	A	(9cis)-retinoic acid	non-polymer		300.4	1	Chemie (9CR)
5	D	2,4-THIAZOLIDIINEDIONE, 5-[[4-[2-(METHYL-2-PYRIDINYLAMINO)ETHOXY]PHENYL]METHYL]-(9CL)	non-polymer		357.4	1	Chemie (BRL)
6		water	water		18.0	231	Chemie (HOH)

Sequence modifications

A: 223 - 462 (UniProt: P19793)

PDB	External Database	Details
Gly 221	-	expression tag
Met 222	-	expression tag
Phe 427	Ser 427	engineered mutation

D: 206 - 477 (UniProt: P37231)

PDB	External Database	Details
Gly 205	-	expression tag

E, F: 676 - 700 (UniProt: Q15788)

PDB	External Database	Details
Ace 675	-	acetylation
Nh2 701	-	amidation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	63916.0
Non-Polymers*	Number of molecules	2
	Total formula weight	657.9
All*	Total formula weight	64573.8

*Water molecules are not included.