5JC9

Structure of the Escherichia coli ribosome with the U1052G mutation in the 16S rRNA

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AA, BA	16S rRNA	polymer	1534	497444.0	2	GenBank (731469900)	Escherichia coli K-12
2	AB, BB	30S ribosomal protein S2	polymer	224	24971.8	2	UniProt (P0A7V0) Pfam (PF00318) In PDB	Escherichia coli K-12
3	AC, BC	30S ribosomal protein S3	polymer	206	23078.8	2	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) In PDB	Escherichia coli K-12
4	AD, BD	30S ribosomal protein S4	polymer	205	23383.0	2	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) In PDB	Escherichia coli K-12
5	AE, BE	30S ribosomal protein S5	polymer	155	16361.9	2	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) In PDB	Escherichia coli K-12
6	AF, BF	30S ribosomal protein S6	polymer	106	12326.3	2	UniProt (P02358) Pfam (PF01250) In PDB	Escherichia coli K-12
7	AG, BG	30S ribosomal protein S7	polymer	151	16861.5	2	UniProt (P02359) Pfam (PF00177) In PDB	Escherichia coli K-12
8	AH, BH	30S ribosomal protein S8	polymer	129	14015.4	2	UniProt (P0A7W7) Pfam (PF00410) In PDB	Escherichia coli K-12
9	AI, BI	30S ribosomal protein S9	polymer	127	14554.9	2	UniProt (P0A7X3) Pfam (PF00380) In PDB	Escherichia coli K-12
10	AJ, BJ	30S ribosomal protein S10	polymer	99	11325.1	2	UniProt (P0A7R5) Pfam (PF00338) In PDB	Escherichia coli K-12
11	AK, BK	30S ribosomal protein S11	polymer	117	12487.2	2	UniProt (P0A7R9) Pfam (PF00411) In PDB	Escherichia coli K-12
12	AL, BL	30S ribosomal protein S12	polymer	123	13683.1	2	UniProt (P0A7S3) Pfam (PF00164) In PDB	Escherichia coli K-12
13	AM, BM	30S ribosomal protein S13	polymer	114	12625.8	2	UniProt (P0A7S9) Pfam (PF00416) In PDB	Escherichia coli K-12
14	AN, BN	30S ribosomal protein S14	polymer	100	11475.4	2	UniProt (P0AG59) Pfam (PF00253) In PDB	Escherichia coli K-12
15	AO, BO	30S ribosomal protein S15	polymer	88	10159.6	2	UniProt (P0ADZ4) Pfam (PF00312) In PDB	Escherichia coli K-12
16	AP, BP	30S ribosomal protein S16	polymer	82	9207.6	2	UniProt (P0A7T3) Pfam (PF00886) In PDB	Escherichia coli K-12
17	AQ, BQ	30S ribosomal protein S17	polymer	80	9263.9	2	UniProt (P0AG63) Pfam (PF00366) In PDB	Escherichia coli K-12
18	AR, BR	30S ribosomal protein S18	polymer	55	6466.5	2	UniProt (P0A7T7) Pfam (PF01084) In PDB	Escherichia coli K-12
19	AS, BS	30S ribosomal protein S19	polymer	79	9057.6	2	UniProt (P0A7U3) Pfam (PF00203) In PDB	Escherichia coli K-12
20	AT, BT	30S ribosomal protein S20	polymer	86	9577.3	2	UniProt (P0A7U7) Pfam (PF01649) In PDB	Escherichia coli K-12
21	AU, BU	30S ribosomal protein S21	polymer	56	6629.7	2	UniProt (P68679) Pfam (PF01165) In PDB	Escherichia coli K-12
22	C1, D1	50S ribosomal protein L32	polymer	56	6332.2	2	UniProt (P0A7N4) Pfam (PF01783) In PDB	Escherichia coli K-12
23	C2, D2	50S ribosomal protein L33	polymer	51	5928.0	2	UniProt (P0A7N9) Pfam (PF00471) In PDB	Escherichia coli K-12
24	C3, D3	50S ribosomal protein L34	polymer	46	5397.5	2	UniProt (P0A7P5) Pfam (PF00468) In PDB	Escherichia coli K-12
25	C4, D4	50S ribosomal protein L35	polymer	64	7181.8	2	UniProt (P0A7Q1) Pfam (PF01632) In PDB	Escherichia coli K-12	Ribosomal protein A
26	C5, D5	50S ribosomal protein L36	polymer	38	4377.4	2	UniProt (P0A7Q6) Pfam (PF00444) In PDB	Escherichia coli K-12	Ribosomal protein B
27	C0, D0	50S ribosomal protein L30	polymer	58	6423.6	2	UniProt (P0AG51) Pfam (PF00327) In PDB	Escherichia coli K-12
28	CB, DB	5S rRNA	polymer	120	38790.1	2	GenBank (939731527)	Escherichia coli K-12
29	CC, DC	50S ribosomal protein L2	polymer	271	29663.2	2	UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB	Escherichia coli K-12
30	CD	50S ribosomal protein L3	polymer	209	22277.5	1	UniProt (P60438) Pfam (PF00297) In PDB	Escherichia coli K-12
31	CA	23S rRNA	polymer	2904	941809.6	1	GenBank (802133627)	Escherichia coli K-12
32	DD	50S ribosomal protein L3	polymer	209	22291.6	1	UniProt (P60438) Pfam (PF00297) In PDB	Escherichia coli K-12
33	CE, DE	50S ribosomal protein L4	polymer	201	22121.6	2	UniProt (P60723) Pfam (PF00573) In PDB	Escherichia coli K-12
34	CF, DF	50S ribosomal protein L5	polymer	177	20073.2	2	UniProt (P62399) Pfam (PF00281) Pfam (PF00673) In PDB	Escherichia coli K-12
35	CG, DG	50S ribosomal protein L6	polymer	176	18801.6	2	UniProt (P0AG55) Pfam (PF00347) In PDB	Escherichia coli K-12
36	CH, DH	50S ribosomal protein L9	polymer	149	15789.0	2	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) In PDB	Escherichia coli K-12
37	CJ, DJ	50S ribosomal protein L11	polymer	134	14020.3	2	UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298) In PDB	Escherichia coli K-12
38	CK, DK	50S ribosomal protein L13	polymer	142	16050.6	2	UniProt (P0AA10) Pfam (PF00572) In PDB	Escherichia coli K-12
39	CL, DL	50S ribosomal protein L14	polymer	123	13565.1	2	UniProt (P0ADY3) Pfam (PF00238) In PDB	Escherichia coli K-12
40	CM, DM	50S ribosomal protein L15	polymer	144	15008.5	2	UniProt (P02413) Pfam (PF00828) In PDB	Escherichia coli K-12
41	CN, DN	50S ribosomal protein L16	polymer	136	15327.3	2	UniProt (P0ADY7) Pfam (PF00252) In PDB	Escherichia coli K-12
42	CO, DO	50S ribosomal protein L17	polymer	125	14193.5	2	UniProt (P0AG44) Pfam (PF01196) In PDB	Escherichia coli K-12
43	CP, DP	50S ribosomal protein L18	polymer	117	12794.7	2	UniProt (P0C018) Pfam (PF00861) In PDB	Escherichia coli K-12
44	CQ, DQ	50S ribosomal protein L19	polymer	114	13028.1	2	UniProt (P0A7K6) Pfam (PF01245) In PDB	Escherichia coli K-12
45	CR, DR	50S ribosomal protein L20	polymer	117	13396.8	2	UniProt (P0A7L3) Pfam (PF00453) In PDB	Escherichia coli K-12
46	CS, DS	50S ribosomal protein L21	polymer	103	11586.4	2	UniProt (P0AG48) Pfam (PF00829) In PDB	Escherichia coli K-12
47	CT, DT	50S ribosomal protein L22	polymer	110	12253.4	2	UniProt (P61175) Pfam (PF00237) In PDB	Escherichia coli K-12
48	CU, DU	50S ribosomal protein L23	polymer	93	10546.5	2	UniProt (P0ADZ0) Pfam (PF00276) In PDB	Escherichia coli K-12
49	CV, DV	50S ribosomal protein L24	polymer	102	11078.9	2	UniProt (P60624) Pfam (PF00467) Pfam (PF17136) In PDB	Escherichia coli K-12
50	CW, DW	50S ribosomal protein L25	polymer	94	10713.5	2	UniProt (P68919) Pfam (PF01386) In PDB	Escherichia coli K-12
51	CX, DX	50S ribosomal protein L27	polymer	76	8275.5	2	UniProt (P0A7L8) Pfam (PF01016) In PDB	Escherichia coli K-12
52	CY, DY	50S ribosomal protein L28	polymer	77	8896.4	2	UniProt (P0A7M2) Pfam (PF00830) In PDB	Escherichia coli K-12
53	CZ, DZ	50S ribosomal protein L29	polymer	62	7155.3	2	UniProt (P0A7M6) Pfam (PF00831) In PDB	Escherichia coli K-12
54	DI	50S ribosomal protein L10	polymer	135	14568.7	1	UniProt (P0A7J3) Pfam (PF00466) In PDB	Escherichia coli K-12	50S ribosomal protein L8
55	DA	23S rRNA	polymer	2904	941811.6	1	GenBank (802133627)	Escherichia coli K-12
56	AA, BA, CA, DB, DA...	MAGNESIUM ION	non-polymer		24.3	469	Chemie (MG)
57	DQ, AA, DR, DA, BA...	TETRAETHYLENE GLYCOL	non-polymer		194.2	7	Chemie (PG4)
58	DA, DE, DK, DN, AA...	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	14	Chemie (MPD)
59	DA, DM, AA	1,4-DIAMINOBUTANE	non-polymer		88.2	16	Chemie (PUT)
60	AB, D5, C5	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
61	D3, AL, DA, DL, DP...	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	11	Chemie (PEG)
62	DA, DB, D1	1,2-ETHANEDIOL	non-polymer		62.1	12	Chemie (EDO)
63	DA, DD, DS, D1, DU...	TRIETHYLENE GLYCOL	non-polymer		150.2	9	Chemie (PGE)
64	DA	SPERMIDINE	non-polymer		145.2	4	Chemie (SPD)
65	DA	PENTAETHYLENE GLYCOL	non-polymer		238.3	2	Chemie (1PE)
66	DA	ACETIC ACID	non-polymer		60.1	3	Chemie (ACY)
67	DA	GUANINE	non-polymer		151.1	1	Chemie (GUN)
68	DA	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
69		water	water		18.0	7746	Chemie (HOH)

Sequence modifications

AA, BA: 1 - 1534 (GenBank: 731469900)

PDB	External Database	Details
G 1052	U 4008236	engineered mutation

CN, DN: 1 - 136 (UniProt: P0ADY7)

PDB	External Database	Details
4d4 81	Arg 81	conflict

DI: 2 - 136 (UniProt: P0A7J3)

PDB	External Database	Details
Val 85	Ser 85	conflict
Thr 86	Met 86	conflict

Contents of the asymmetric unit

Polymers	Number of chains	105
	Total formula weight	4250210.7
Non-Polymers*	Number of molecules	552
	Total formula weight	20794.4
All*	Total formula weight	4271005.1

*Water molecules are not included.