5JC7
Crystal structure of chicken MDA5 with 5'p 24-mer dsRNA and ADP-Mg2+ at 2.75 A resolution.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Melanoma differentiation associated protein-5 | polymer | 701 | 81667.0 | 2 | UniProt (D9N195) Pfam (PF04851) Pfam (PF18119) Pfam (PF00271) Pfam (PF11648) | Gallus gallus (Chicken) | |
| 2 | C (X) | RNA (5'-R(P*GP*GP*AP*CP*GP*UP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*AP*CP*GP*UP*CP*CP*CP*C)-3') | polymer | 25 | 8004.8 | 1 | synthetic construct | ||
| 3 | D (Y) | RNA (5'-R(P*GP*GP*GP*AP*CP*GP*UP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*AP*CP*GP*UP*CP*CP*C)-3') | polymer | 24 | 7699.6 | 1 | synthetic construct | ||
| 4 | E, H (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 5 | F, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G, J (A, B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) |
Sequence modifications
A, B: 298 - 994 (UniProt: D9N195)
| PDB | External Database | Details |
|---|---|---|
| Gly 294 | - | expression tag |
| Ala 295 | - | expression tag |
| Met 296 | - | expression tag |
| Gly 297 | - | expression tag |
| Gln 436 | Glu 436 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 179038.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1033.8 | |
| All* | Total formula weight | 180072.3 |
