5J3N

C-terminal domain of EcoR124I HsdR subunit fused with the pH-sensitive GFP variant ratiometric pHluorin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Green fluorescent protein,HsdR	polymer	398	45491.3	2	UniProt (P42212) UniProt (Q304R3) Pfam (PF01353) Pfam (PF12008) UniProt (by SIFTS) (P10486) In PDB	Aequorea victoria (Jellyfish)
2		water	water		18.0	198	Chemie (HOH)

Sequence modifications

A, B: 8 - 244 (UniProt: P42212)

PDB	External Database	Details
Met 1	-	initiating methionine
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gys 72	Ser 65	chromophore
Gys 72	Tyr 66	chromophore
Gys 72	Gly 67	chromophore
Arg 86	Gln 80	engineered mutation
Asp 138	Glu 132	engineered mutation
Glu 153	Ser 147	engineered mutation
Leu 155	Asn 149	engineered mutation
Thr 167	Ile 161	engineered mutation
Ala 169	Val 163	engineered mutation
Ile 170	Asn 164	engineered mutation
Gln 172	Lys 166	engineered mutation
Val 173	Ile 167	engineered mutation
His 174	Arg 168	engineered mutation
Gly 181	Ser 175	engineered mutation
His 208	Ser 202	engineered mutation
Glu 245	-	linker
Phe 246	-	linker

A, B: 247 - 398 (UniProt: Q304R3)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	90982.6
All*	Total formula weight	90982.6

*Water molecules are not included.