5J3N
C-terminal domain of EcoR124I HsdR subunit fused with the pH-sensitive GFP variant ratiometric pHluorin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9763 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.386, 176.505, 126.023 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.450 |
R-factor | 0.1949 |
Rwork | 0.192 |
R-free | 0.25383 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w7s |
RMSD bond length | 0.014 |
RMSD bond angle | 1.729 |
Data reduction software | XDS (Oct 15, 2015) |
Phasing software | MOLREP (11.4.04) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.101 | 0.510 |
Number of reflections | 34464 | |
<I/σ(I)> | 11.7 | 2.74 |
Completeness [%] | 99.2 | 97.4 |
Redundancy | 4.68 | 4.63 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 20% (w/v) PEG 3350, 0.2 M KH2PO4, 4% v/v acetone |