5J2U

Tubulin-MMAF complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D (B, D)	Tubulin beta-2B chain	polymer	445	49999.9	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)
3	E (E)	Stathmin-4	polymer	143	16844.2	1	UniProt (P63043) Pfam (PF00836)	Rattus norvegicus (Rat)	Stathmin-like protein B3,RB3
4	F (F)	Tubulin beta-2B chain	polymer	384	44378.5	1	UniProt (E1BQ43) Pfam (PF03133) UniProt (by SIFTS) (P38584)	Gallus gallus (Chicken)
5	G, H (G, H)	Monomethyl auristatin F (MMAF)	polymer	5	732.0	2		synthetic construct
6	I, O (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
7	J, M, N, P, Q (A, B, C)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
8	K (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
9	L, R (B, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
10	S (F)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
11	T, U, V, W, X... (A, B, C, D, E...)	water	water		18.0	138	Chemie (HOH)

Sequence modifications

E: 5 - 145 (UniProt: P63043)

PDB	External Database	Details
Met 3	-	initiating methionine
Ala 4	-	expression tag

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	263095.2
Non-Polymers*	Number of molecules	11
	Total formula weight	2599.6
All*	Total formula weight	265694.8

*Water molecules are not included.