5J12

Structure of human TSLP:TSLPR in complex with mouse IL-7Ralpha

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Thymic stromal lymphopoietin	polymer	163	18522.5	1	UniProt (Q969D9) Pfam (PF15216)	Homo sapiens (Human)
2	B (B)	Interleukin-7 receptor subunit alpha	polymer	223	25431.8	1	UniProt (P16872) Pfam (PF18447)	Mus musculus (Mouse)	IL-7RA
3	C (C)	Cytokine receptor-like factor 2	polymer	230	26559.5	1	UniProt (Q9HC73) Pfam (PF22012) Pfam (PF21605)	Homo sapiens (Human)	Cytokine receptor-like 2,IL-XR,Thymic stromal lymphopoietin protein receptor,TSLP receptor
4	D, H, I (A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
5	E, F, G (C)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	J, K (B, C)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 1 - 159 (UniProt: Q969D9)

PDB	External Database	Details
Gln 64	Asn 64	engineered mutation
-	Lys 126	deletion
-	Arg 127	deletion
-	Arg 128	deletion
-	Lys 129	deletion
-	Arg 130	deletion
Ser 131	Lys 131	engineered mutation
Gly 160	-	expression tag
Thr 161	-	expression tag
Lys 162	-	expression tag
His 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag

B: 21 - 239 (UniProt: P16872)

PDB	External Database	Details
Gly 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
Met 20	-	expression tag

C: 1 - 221 (UniProt: Q9HC73)

PDB	External Database	Details
Gly 222	-	expression tag
Thr 223	-	expression tag
Lys 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	70513.8
Non-Polymers*	Number of molecules	6
	Total formula weight	951.8
All*	Total formula weight	71465.7

*Water molecules are not included.