5J12
Structure of human TSLP:TSLPR in complex with mouse IL-7Ralpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 125.442, 125.442, 194.573 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.430 - 3.550 |
R-factor | 0.2145 |
Rwork | 0.214 |
R-free | 0.22530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 4NN5 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.190 |
Data reduction software | XDS (VERSION January 10, 2014) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.760 |
High resolution limit [Å] | 3.550 | 3.550 |
Rmeas | 0.177 | 1.122 |
Number of reflections | 11341 | |
<I/σ(I)> | 10.8 | 2 |
Completeness [%] | 98.4 | 97.9 |
Redundancy | 10.7 | 10.5 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.53 M ammonium sulfate 0.1 M sodium chloride 0.1 M BIS-TRIS pH 7.0 |