5J11

Structure of human TSLP in complex with TSLPR and IL-7Ralpha

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Thymic stromal lymphopoietin	polymer	147	16523.9	1	UniProt (Q969D9) Pfam (PF15216)	Homo sapiens (Human)
2	B (B)	Interleukin-7 receptor subunit alpha	polymer	240	27592.2	1	UniProt (D6RGV2) Pfam (PF18447) Pfam (PF00041) UniProt (by SIFTS) (P16871)	Homo sapiens (Human)
3	C (C)	Cytokine receptor-like factor 2	polymer	230	26573.6	1	UniProt (Q9HC73) Pfam (PF22012) Pfam (PF21605)	Homo sapiens (Human)	Cytokine receptor-like 2,IL-XR,Thymic stromal lymphopoietin protein receptor,TSLP receptor
4	D (C)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
5	E, F (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
6	G, H, I (A, B, C)	water	water		18.0	29	Chemie (HOH)

Sequence modifications

A: 29 - 159 (UniProt: Q969D9)

PDB	External Database	Details
Met 8	-	initiating methionine
Gly 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
Ser 18	-	expression tag
Ser 19	-	expression tag
Gly 20	-	expression tag
Leu 21	-	expression tag
Val 22	-	expression tag
Pro 23	-	expression tag
Arg 24	-	expression tag
Gly 25	-	expression tag
Ser 26	-	expression tag
His 27	-	expression tag
Met 28	-	expression tag
-	Lys 126	deletion
-	Arg 127	deletion
-	Arg 128	deletion
-	Lys 129	deletion
-	Arg 130	deletion

B: 21 - 236 (UniProt: D6RGV2)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag
Ser 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
Gly 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Pro 15	-	expression tag
Arg 16	-	expression tag
Gly 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
Met 20	-	expression tag
Glu 237	-	expression tag
Met 238	-	expression tag
Asp 239	-	expression tag

C: 1 - 221 (UniProt: Q9HC73)

PDB	External Database	Details
Gln 47	Asn 47	conflict
Gly 222	-	expression tag
Thr 223	-	expression tag
Lys 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	70689.6
Non-Polymers*	Number of molecules	3
	Total formula weight	592.6
All*	Total formula weight	71282.2

*Water molecules are not included.