5J11
Structure of human TSLP in complex with TSLPR and IL-7Ralpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.783, 66.644, 91.971 |
Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
Resolution | 45.760 - 2.560 |
R-factor | 0.1926 |
Rwork | 0.191 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | X-ray structure of human TSLP in complex with human TSLPR and mouse IL-7Ralpha; PDB 3DI2: chain B |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS (VERSION November 3, 2014) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.720 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmeas | 0.062 | 0.776 |
Number of reflections | 24638 | |
<I/σ(I)> | 14.2 | 1.7 |
Completeness [%] | 97.8 | 94.2 |
Redundancy | 3.2 | 3.1 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 0.02 M Citric acid 0.08 M BIS-TRIS propane 16% w/v polyethylene glycol 3350 |