5J11

Structure of human TSLP in complex with TSLPR and IL-7Ralpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	C 1 2 1
Unit cell lengths	135.783, 66.644, 91.971
Unit cell angles	90.00, 109.20, 90.00

Refinement procedure

Resolution	45.760 - 2.560
R-factor	0.1926
Rwork	0.191
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	X-ray structure of human TSLP in complex with human TSLPR and mouse IL-7Ralpha; PDB 3DI2: chain B
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	XDS (VERSION November 3, 2014)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.560	2.560
Rmeas	0.062	0.776
Number of reflections	24638
<I/σ(I)>	14.2	1.7
Completeness [%]	97.8	94.2
Redundancy	3.2	3.1
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	0.02 M Citric acid 0.08 M BIS-TRIS propane 16% w/v polyethylene glycol 3350