5ITZ
Crystal structure of the SAC domain of CPAP in a complex with Tubulin and Darpin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 1 | UniProt (P81947) Pfam (PF00091) Pfam (PF03953) | Bos taurus (Bovine) | Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain |
| 2 | B (B) | Tubulin beta-2B chain | polymer | 445 | 49999.9 | 1 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) | Bos taurus (Bovine) | |
| 3 | C (D) | Centromere protein J | polymer | 129 | 14889.8 | 1 | UniProt (Q9HC77) Pfam (PF25779) | Homo sapiens (Human) | CENP-J,Centrosomal P4.1-associated protein,LAG-3-associated protein,LYST-interacting protein 1 |
| 4 | D (F) | Designed ankyrin repeat protein (DARPIN) D1 | polymer | 127 | 13355.3 | 1 | synthetic construct | ||
| 5 | E (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 7 | G (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 8 | H (B) | N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide | non-polymer | 399.4 | 1 | Chemie (LOC) PubChem (6167) PubChem (53278) PubChem (2833) | |||
| 9 | I, J, K, L (A, B, D, F) | water | water | 18.0 | 473 | Chemie (HOH) |
Sequence modifications
D: 311 - 422 (UniProt: Q9HC77)
| PDB | External Database | Details |
|---|---|---|
| Met 294 | - | initiating methionine |
| Ala 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
| Gly 302 | - | expression tag |
| Ser 303 | - | expression tag |
| Leu 304 | - | expression tag |
| Val 305 | - | expression tag |
| Pro 306 | - | expression tag |
| Arg 307 | - | expression tag |
| Gly 308 | - | expression tag |
| Ser 309 | - | expression tag |
| Ala 310 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 128449.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1390.1 | |
| All* | Total formula weight | 129839.5 |
