5ITZ

Crystal structure of the SAC domain of CPAP in a complex with Tubulin and Darpin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tubulin alpha-1B chain	polymer	451	50204.4	1	UniProt (P81947) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (Bovine)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B	Tubulin beta-2B chain	polymer	445	49999.9	1	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (Bovine)
3	D	Centromere protein J	polymer	129	14889.8	1	UniProt (Q9HC77) In PDB	Homo sapiens (Human)	CENP-J,Centrosomal P4.1-associated protein,LAG-3-associated protein,LYST-interacting protein 1
4	F	Designed ankyrin repeat protein (DARPIN) D1	polymer	127	13355.3	1		synthetic construct
5	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
6	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
8	B	N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide	non-polymer		399.4	1	Chemie (LOC)
9		water	water		18.0	473	Chemie (HOH)

Sequence modifications

D: 311 - 422 (UniProt: Q9HC77)

PDB	External Database	Details
Met 294	-	initiating methionine
Ala 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
Gly 302	-	expression tag
Ser 303	-	expression tag
Leu 304	-	expression tag
Val 305	-	expression tag
Pro 306	-	expression tag
Arg 307	-	expression tag
Gly 308	-	expression tag
Ser 309	-	expression tag
Ala 310	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128449.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1390.1
All*	Total formula weight	129839.5

*Water molecules are not included.