5ITZ
Crystal structure of the SAC domain of CPAP in a complex with Tubulin and Darpin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 1 | UniProt (P81947) Pfam (PF00091) Pfam (PF03953) | Bos taurus (Bovine) | Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain |
| 2 | B (B) | Tubulin beta-2B chain | polymer | 445 | 49999.9 | 1 | UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) | Bos taurus (Bovine) | |
| 3 | C (D) | Centromere protein J | polymer | 129 | 14889.8 | 1 | UniProt (Q9HC77) | Homo sapiens (Human) | CENP-J,Centrosomal P4.1-associated protein,LAG-3-associated protein,LYST-interacting protein 1 |
| 4 | D (F) | Designed ankyrin repeat protein (DARPIN) D1 | polymer | 127 | 13355.3 | 1 | synthetic construct | ||
| 5 | E (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | G (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 8 | H (B) | N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide | non-polymer | 399.4 | 1 | Chemie (LOC) | |||
| 9 | I, J, K, L (A, B, D, F) | water | water | 18.0 | 473 | Chemie (HOH) |
Sequence modifications
D: 311 - 422 (UniProt: Q9HC77)
| PDB | External Database | Details |
|---|---|---|
| Met 294 | - | initiating methionine |
| Ala 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
| Gly 302 | - | expression tag |
| Ser 303 | - | expression tag |
| Leu 304 | - | expression tag |
| Val 305 | - | expression tag |
| Pro 306 | - | expression tag |
| Arg 307 | - | expression tag |
| Gly 308 | - | expression tag |
| Ser 309 | - | expression tag |
| Ala 310 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 128449.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1390.1 | |
| All* | Total formula weight | 129839.5 |
