5ITZ
Crystal structure of the SAC domain of CPAP in a complex with Tubulin and Darpin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-17 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.080, 85.340, 98.690 |
Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
Resolution | 52.644 - 2.200 |
R-factor | 0.1789 |
Rwork | 0.177 |
R-free | 0.21740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4drx |
RMSD bond length | 0.008 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.500 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.078 | 0.707 |
Number of reflections | 51519 | |
<I/σ(I)> | 18.11 | |
Completeness [%] | 99.9 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG 550 mono methyl ether (MME) and 0.1 M MES, pH 6.5 |