5IRC
p190A GAP domain complex with RhoA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Rho GTPase-activating protein 35 | polymer | 201 | 23307.5 | 2 | UniProt (P81128) Pfam (PF00620) In PDB | Rattus norvegicus (Rat) | GAP-associated protein p190,Glucocorticoid receptor DNA-binding factor 1 |
| 2 | F, D | Transforming protein RhoA | polymer | 186 | 20819.8 | 2 | UniProt (P61586) Pfam (PF00071) In PDB | Homo sapiens (Human) | Rho cDNA clone 12,h12 |
| 3 | A, B | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 4 | F, D | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 5 | F, D | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | F, D | TRIFLUOROMAGNESATE | non-polymer | 81.3 | 2 | Chemie (MGF) | |||
| 7 | water | water | 18.0 | 1019 | Chemie (HOH) |
Sequence modifications
A, B: 1242 - 1439 (UniProt: P81128)
F, D: 2 - 181 (UniProt: P61586)
| PDB | External Database | Details |
|---|---|---|
| Ala 1239 | - | expression tag |
| Gly 1240 | - | expression tag |
| Ser 1241 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | cloning artifact |
| Ala -3 | - | cloning artifact |
| Met -2 | - | cloning artifact |
| Gly -1 | - | cloning artifact |
| Ser 0 | - | cloning artifact |
| Pro 1 | - | cloning artifact |
| Asn 25 | Phe 25 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 88254.5 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1204.0 | |
| All* | Total formula weight | 89458.5 |
