5IRC

p190A GAP domain complex with RhoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Rho GTPase-activating protein 35	polymer	201	23307.5	2	UniProt (P81128) Pfam (PF00620)	Rattus norvegicus (Rat)	GAP-associated protein p190,Glucocorticoid receptor DNA-binding factor 1
2	B, D (F, D)	Transforming protein RhoA	polymer	186	20819.8	2	UniProt (P61586) Pfam (PF00071)	Homo sapiens (Human)	Rho cDNA clone 12,h12
3	E, F, J (A, B)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL) PubChem (312)
4	G, K (F, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
5	H, L (F, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
6	I, M (F, D)	TRIFLUOROMAGNESATE	non-polymer		81.3	2	Chemie (MGF)
7	N, O, P, Q (A, F, B, D)	water	water		18.0	1019	Chemie (HOH)

Sequence modifications

A, B: 1242 - 1439 (UniProt: P81128)

PDB	External Database	Details
Ala 1239	-	expression tag
Gly 1240	-	expression tag
Ser 1241	-	expression tag

F, D: 2 - 181 (UniProt: P61586)

PDB	External Database	Details
Gly -4	-	cloning artifact
Ala -3	-	cloning artifact
Met -2	-	cloning artifact
Gly -1	-	cloning artifact
Ser 0	-	cloning artifact
Pro 1	-	cloning artifact
Asn 25	Phe 25	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88254.5
Non-Polymers*	Number of molecules	9
	Total formula weight	1204.0
All*	Total formula weight	89458.5

*Water molecules are not included.