5IQN

Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_SRIRIRGYVR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, G)	Protein FimG	polymer	132	13666.8	2	UniProt (P08190)	Escherichia coli K-12
2	B, C (B, F)	Protein FimF	polymer	10	1279.5	2	UniProt (P08189)	Escherichia coli K-12	DsF_SRIRIRGYVR
3	E, F (A, G)	COBALT (II) ION	non-polymer		58.9	2	Chemie (CO)
4	G (G)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	H, I, J, K (A, B, F, G)	water	water		18.0	486	Chemie (HOH)

Sequence modifications

A, G: 13 - 144 (UniProt: P08190)

PDB	External Database	Details
Glu 134	Gln 157	engineered mutation

B, F: 1 - 10 (UniProt: P08189)

PDB	External Database	Details
Arg 2	Thr 26	engineered mutation
Arg 4	Thr 28	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	29892.7
Non-Polymers*	Number of molecules	3
	Total formula weight	179.9
All*	Total formula weight	30072.7

*Water molecules are not included.