5IQN
Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_SRIRIRGYVR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-01 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.8 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.680, 52.880, 83.140 |
Unit cell angles | 90.00, 98.71, 90.00 |
Refinement procedure
Resolution | 25.384 - 1.000 |
R-factor | 0.1193 |
Rwork | 0.118 |
R-free | 0.14600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bfw |
RMSD bond length | 0.013 |
RMSD bond angle | 1.419 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.980 |
High resolution limit [Å] | 0.960 | 0.960 |
Rmerge | 0.064 | 1.304 |
Rpim | 0.064 | 1.304 |
Number of reflections | 133060 | |
<I/σ(I)> | 12.9 | |
Completeness [%] | 99.3 | |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | MOPS-NaOH pH 6.8 (RT), PEG-1500, CoCl2, NaCl |