5IPV

Cryo-EM structure of GluN1/GluN2B NMDA receptor in the DCKA/D-APV-bound conformation, state 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	N-methyl-D-aspartate receptor subunit NR1-8a	polymer	822	92651.2	2	UniProt (C0KD18) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) Pfam (PF00497) UniProt (by SIFTS) (A0A1L8F5J9) In PDB	Xenopus laevis (African clawed frog)
2	B, D	Ionotropic glutamate receptor subunit NR2B	polymer	825	93234.7	2	UniProt (A7XY94) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) Pfam (PF00497) In PDB	Xenopus laevis (African clawed frog)

Sequence modifications

A, C: 23 - 828 (UniProt: C0KD18)

PDB	External Database	Details
Phe 51	Lys 51	engineered mutation
Phe 52	Arg 52	engineered mutation
Gln 300	Asn 300	engineered mutation
Gln 350	Asn 350	engineered mutation
Asp 368	Asn 368	engineered mutation
Asp 440	Asn 440	engineered mutation
Asp 469	Asn 469	engineered mutation
Ala 493	Lys 493	engineered mutation
Ala 494	Lys 494	engineered mutation
Ala 495	Glu 495	engineered mutation
-	Lys 588	deletion
-	Val 589	deletion
-	Asn 590	deletion
-	Ser 591	deletion
-	Glu 592	deletion
-	Glu 593	deletion
-	Glu 594	deletion
-	Glu 595	deletion
Arg 602	Gly 610	engineered mutation
Leu 609	Ile 617	engineered mutation
Arg 648	Asp 656	engineered mutation
Glu 761	Asn 769	engineered mutation
Ser 829	-	expression tag
Arg 830	-	expression tag
Ala 831	-	expression tag
Glu 832	-	expression tag
Ala 833	-	expression tag
Lys 834	-	expression tag
Arg 835	-	expression tag
Met 836	-	expression tag
Lys 837	-	expression tag
Gly 838	-	expression tag
Leu 839	-	expression tag
Glu 840	-	expression tag
Val 841	-	expression tag
Leu 842	-	expression tag
Phe 843	-	expression tag
Gln 844	-	expression tag

B, D: 1 - 825 (UniProt: A7XY94)

PDB	External Database	Details
Ser 20	Met 20	engineered mutation
Arg 21	Gly 21	engineered mutation
Ala 22	Cys 22	engineered mutation
Glu 64	Ala 64	engineered mutation
Gln 69	Asn 69	engineered mutation
Asp 343	Asn 343	engineered mutation
-	Lys 382	deletion
-	Tyr 383	deletion
-	Tyr 384	deletion
-	Val 385	deletion
Val 486	Thr 490	engineered mutation
-	Arg 584	deletion
-	Cys 585	deletion
-	Leu 586	deletion
-	Ala 587	deletion
-	Asp 588	deletion
-	Gly 589	deletion
-	Arg 590	deletion
-	Glu 591	deletion
-	Pro 592	deletion
-	Gly 593	deletion
Leu 601	Val 615	engineered mutation
Arg 640	Glu 654	engineered mutation
Arg 641	Glu 655	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	371772.0
All*	Total formula weight	371772.0

*Water molecules are not included.