5IPP

Structure of Bacillus NanoRNase A active site mutant bound to a mononucleotide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Bifunctional oligoribonuclease and PAP phosphatase NrnA	polymer	333	37416.8	4	UniProt (O34600) Pfam (PF01368) Pfam (PF02272)	Bacillus subtilis (strain 168)	3'(2'),5'-bisphosphate nucleotidase,3'-phosphoadenosine 5'-phosphate phosphatase,PAP phosphatase,nanoRNase
2	E, F (A, C)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
3	G, H, I, J (A, B, C, D)	water	water		18.0	760	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 313 (UniProt: O34600)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Ser -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag
Ala 103	His 103	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	149667.2
Non-Polymers*	Number of molecules	2
	Total formula weight	694.4
All*	Total formula weight	150361.6

*Water molecules are not included.