5IPP
Structure of Bacillus NanoRNase A active site mutant bound to a mononucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.031, 125.193, 116.818 |
Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
Resolution | 46.820 - 1.950 |
R-factor | 0.18812 |
Rwork | 0.186 |
R-free | 0.23761 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.885 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.820 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.074 | |
Number of reflections | 98753 | |
<I/σ(I)> | 16.44 | |
Completeness [%] | 97.6 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | PEG MME 2000, sodium acetate, ammonium acetate, glycerol |