5IM6

Crystal structure of designed two-component self-assembling icosahedral cage I32-28

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, AA, B, BA, C... (A, B, C, D, E...)	Designed self-assembling icosahedral cage I32-28 trimeric subunit	polymer	157	16798.3	20	UniProt (Q2SZ09) Pfam (PF01923)	Burkholderia thailandensis E264	Designed ATP:cob(I)alamin adenosyltransferase
2	EA, FA, GA, HA, IA... (U, V, W, X, Y...)	Designed self-assembling icosahedral cage I32-28 dimeric subunit	polymer	165	17387.7	20	UniProt (Q9RSW5) Pfam (PF12723)	Deinococcus radiodurans R1

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T: 29 - 183 (UniProt: Q2SZ09)

PDB	External Database	Details
Met 27	-	expression tag
Gly 28	-	expression tag
Glu 86	Asp 86	engineered mutation
Asp 87	Ala 87	engineered mutation
Leu 90	Ala 90	engineered mutation
Ala 93	Asp 93	engineered mutation
Leu 94	Gly 94	engineered mutation
Val 97	Ala 97	engineered mutation
Lys 136	Val 136	engineered mutation
Ile 146	Ala 146	engineered mutation
Ala 149	Arg 149	engineered mutation
Ala 150	Arg 150	engineered mutation
Leu 154	Arg 154	engineered mutation

U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n: 1 - 157 (UniProt: Q9RSW5)

PDB	External Database	Details
Gln 35	Arg 35	engineered mutation
Arg 36	Phe 36	engineered mutation
Arg 54	Thr 54	engineered mutation
Glu 122	Trp 122	engineered mutation
Val 129	Lys 129	engineered mutation
Val 137	Arg 137	engineered mutation
Leu 140	Glu 140	engineered mutation
Leu 141	Arg 141	engineered mutation
Met 144	Lys 144	engineered mutation
Gln 148	Glu 148	engineered mutation
Leu 158	-	expression tag
Glu 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
His 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	40
	Total formula weight	683719.8
All*	Total formula weight	683719.8

*Water molecules are not included.