5IIT

Structure of SPX domain of the yeast inorganic polyphophate polymerase Vtc4 crystallized by carrier-driven crystallization in fusion with the macro domain of human histone macroH2A1.1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Vacuolar transporter chaperone 4,Core histone macro-H2A.1	polymer	374	42234.9	4	UniProt (P47075) UniProt (O75367) Pfam (PF01661) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Phosphate metabolism protein 3,mH2A1,Histone H2A.y,H2A/y,Medulloblastoma antigen MU-MB-50.205
2	A, B, C, D	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	4	Chemie (MES)
3	A, B, C, D	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	A, B, C	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5	B, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6		water	water		18.0	184	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 178 (UniProt: P47075)

PDB	External Database	Details
Ala 179	-	linker
Gly 180	-	linker
Ser 181	-	linker

A, B, C, D: 182 - 367 (UniProt: O75367)

PDB	External Database	Details
Glu 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	168939.7
Non-Polymers*	Number of molecules	15
	Total formula weight	1524.1
All*	Total formula weight	170463.9

*Water molecules are not included.