5IIT
Structure of SPX domain of the yeast inorganic polyphophate polymerase Vtc4 crystallized by carrier-driven crystallization in fusion with the macro domain of human histone macroH2A1.1
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 8 |
| Details | binding site for residue MES A 401 |
| Chain | Residue |
| A | PRO216 |
| A | THR217 |
| A | ASN218 |
| A | GLY313 |
| A | GLY315 |
| A | ARG316 |
| A | ASN317 |
| A | EDO403 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 A 402 |
| Chain | Residue |
| A | LYS132 |
| A | LYS129 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 403 |
| Chain | Residue |
| A | GLY225 |
| A | GLU226 |
| A | VAL227 |
| A | ARG316 |
| A | MES401 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 404 |
| Chain | Residue |
| A | TYR124 |
| A | LYS157 |
| A | GLU158 |
| A | ASN159 |
| A | TYR160 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 401 |
| Chain | Residue |
| B | TYR124 |
| B | LYS157 |
| B | GLU158 |
| B | ASN159 |
| B | TYR160 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | binding site for residue MES B 402 |
| Chain | Residue |
| B | PRO216 |
| B | THR217 |
| B | GLY224 |
| B | ILE312 |
| B | GLY313 |
| B | GLY315 |
| B | ARG316 |
| B | ASN317 |
| B | SO4403 |
| C | ARG316 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 B 403 |
| Chain | Residue |
| B | GLY225 |
| B | GLU226 |
| B | GLY315 |
| B | ARG316 |
| B | MES402 |
| B | HOH541 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue MG B 404 |
| Chain | Residue |
| B | GLY263 |
| B | LEU266 |
| B | ALA268 |
| B | HOH543 |
| B | HOH551 |
| site_id | AC9 |
| Number of Residues | 13 |
| Details | binding site for residue MES C 401 |
| Chain | Residue |
| B | TYR222 |
| C | PRO216 |
| C | THR217 |
| C | ASN218 |
| C | TYR222 |
| C | SER276 |
| C | ILE312 |
| C | GLY313 |
| C | GLY315 |
| C | ARG316 |
| C | ASN317 |
| C | SO4402 |
| C | EDO403 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 C 402 |
| Chain | Residue |
| B | ARG316 |
| C | GLU226 |
| C | GLY315 |
| C | ARG316 |
| C | MES401 |
| C | EDO403 |
| site_id | AD2 |
| Number of Residues | 9 |
| Details | binding site for residue EDO C 403 |
| Chain | Residue |
| C | HIS215 |
| C | PRO216 |
| C | TYR222 |
| C | GLY225 |
| C | GLU226 |
| C | VAL227 |
| C | GLY228 |
| C | MES401 |
| C | SO4402 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue EDO C 404 |
| Chain | Residue |
| C | GLN42 |
| C | GLU45 |
| C | THR46 |
| C | PHE140 |
| site_id | AD4 |
| Number of Residues | 5 |
| Details | binding site for residue MG C 405 |
| Chain | Residue |
| C | GLY263 |
| C | LEU266 |
| C | ALA268 |
| C | LYS269 |
| C | HOH544 |
| site_id | AD5 |
| Number of Residues | 7 |
| Details | binding site for residue MES D 401 |
| Chain | Residue |
| D | PRO216 |
| D | THR217 |
| D | TYR222 |
| D | GLY313 |
| D | GLY315 |
| D | ARG316 |
| D | ASN317 |
| site_id | AD6 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 D 402 |
| Chain | Residue |
| C | THR189 |
| C | LYS190 |
| D | SER188 |
| D | THR189 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 32 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"A0A0D2UG83","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"17081983","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22106047","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 549 |
| Details | Domain: {"description":"Macro","evidences":[{"source":"PROSITE-ProRule","id":"PRU00490","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 28 |
| Details | Region: {"description":"Important for inositol polyphosphate binding","evidences":[{"source":"PubMed","id":"27080106","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 8 |
| Details | Site: {"description":"Important for inositol polyphosphate binding","evidences":[{"source":"UniProtKB","id":"P43585","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
