5I69

MBP-MamC magnetite-interaction component mutant-D70A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose-binding periplasmic protein,Tightly bound bacterial magnetic particle protein,Maltose-binding periplasmic protein	polymer	412	45146.9	1	UniProt (P0AEX9) UniProt (Q2W8S0) Pfam (PF01547)	Escherichia coli	MBP,MMBP,Maltodextrin-binding protein,MBP,MMBP,Maltodextrin-binding protein
2	B (B)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	C (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	D (A)	water	water		18.0	69	Chemie (HOH)

Sequence modifications

A: 1 - 363 (UniProt: P0AEX9)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

A: 364 - 384 (UniProt: Q2W8S0)

PDB	External Database	Details
Ala 377	Asp 70	engineered mutation

A: 385 - 391 (UniProt: P0AEX9)

PDB	External Database	Details
Asn 388	-	conflict
Ser 389	-	conflict
Ser 390	-	conflict
Ser 391	-	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45146.9
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	1
	Total formula weight	96.1
All*	Total formula weight	45585.3

*Water molecules are not included.