5I69
MBP-MamC magnetite-interaction component mutant-D70A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.236, 67.236, 209.019 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.010 - 2.700 |
| R-factor | 0.20859 |
| Rwork | 0.204 |
| R-free | 0.29284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3puz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.584 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.010 | |
| High resolution limit [Å] | 2.700 | |
| Rmerge | 0.098 | 0.297 |
| Number of reflections | 13928 | |
| <I/σ(I)> | 44.02 | |
| Completeness [%] | 90.3 | |
| Redundancy | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2M ammonium sulfate and 0.1M sodium citrate pH 4.5 |
