5I4N

Crystal Structure of the E596A V617F Mutant JAK2 Pseudokinase Domain Bound to Mg-ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tyrosine-protein kinase JAK2	polymer	290	33242.2	1	UniProt (O60674) Pfam (PF07714)	Homo sapiens (Human)	Janus kinase 2,JAK-2
2	B (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	E (A)	water	water		18.0	186	Chemie (HOH)

Sequence modifications

A: 535 - 816 (UniProt: O60674)

PDB	External Database	Details
Ala 596	Glu 596	engineered mutation
Phe 617	Val 617	engineered mutation
Ala 659	Trp 659	engineered mutation
Ala 777	Trp 777	engineered mutation
His 794	Phe 794	engineered mutation
Leu 817	-	expression tag
Val 818	-	expression tag
Pro 819	-	expression tag
Arg 820	-	expression tag
Gly 821	-	expression tag
Ser 822	-	expression tag
His 823	-	expression tag
His 824	-	expression tag
His 825	-	expression tag
His 826	-	expression tag
His 827	-	expression tag
His 828	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33242.2
Non-Polymers*	Number of molecules	3
	Total formula weight	623.6
All*	Total formula weight	33865.7

*Water molecules are not included.