5HVK

Crystal structure of LIMK1 mutant D460N in complex with full-length cofilin-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	LIM domain kinase 1	polymer	315	36473.2	2	UniProt (P53667) Pfam (PF07714) In PDB	Homo sapiens (Human)	LIMK-1
2	B	Cofilin-1	polymer	165	18511.3	1	UniProt (P23528) Pfam (PF00241) In PDB	Homo sapiens (Human)	18 kDa phosphoprotein,p18,Cofilin,non-muscle isoform
3	D	Cofilin-1	polymer	165	18431.4	1	UniProt (P23528) Pfam (PF00241) In PDB	Homo sapiens (Human)	18 kDa phosphoprotein,p18,Cofilin,non-muscle isoform
4	A, C	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
5	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, C: 329 - 638 (UniProt: P53667)

PDB	External Database	Details
Gly 324	-	expression tag
Ala 325	-	expression tag
Met 326	-	expression tag
Gly 327	-	expression tag
Ser 328	-	expression tag
Asn 460	Asp 460	engineered mutation

B: 2 - 166 (UniProt: P23528)

PDB	External Database	Details
Thr 69	Ala 69	engineered mutation

D: 2 - 166 (UniProt: P23528)

PDB	External Database	Details
Thr 69	Ala 69	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	109889.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.0
All*	Total formula weight	110950.1

*Water molecules are not included.