5HVK
Crystal structure of LIMK1 mutant D460N in complex with full-length cofilin-1
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006468 | biological_process | protein phosphorylation |
| B | 0000281 | biological_process | mitotic cytokinesis |
| B | 0003779 | molecular_function | actin binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005615 | cellular_component | extracellular space |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005856 | cellular_component | cytoskeleton |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0005925 | cellular_component | focal adhesion |
| B | 0007010 | biological_process | cytoskeleton organization |
| B | 0007266 | biological_process | Rho protein signal transduction |
| B | 0009615 | biological_process | response to virus |
| B | 0015629 | cellular_component | actin cytoskeleton |
| B | 0016020 | cellular_component | membrane |
| B | 0016363 | cellular_component | nuclear matrix |
| B | 0022604 | biological_process | regulation of cell morphogenesis |
| B | 0030027 | cellular_component | lamellipodium |
| B | 0030036 | biological_process | actin cytoskeleton organization |
| B | 0030042 | biological_process | actin filament depolymerization |
| B | 0030043 | biological_process | actin filament fragmentation |
| B | 0030424 | cellular_component | axon |
| B | 0030426 | cellular_component | growth cone |
| B | 0031258 | cellular_component | lamellipodium membrane |
| B | 0031982 | cellular_component | vesicle |
| B | 0032587 | cellular_component | ruffle membrane |
| B | 0040019 | biological_process | positive regulation of embryonic development |
| B | 0042995 | cellular_component | cell projection |
| B | 0043066 | biological_process | negative regulation of apoptotic process |
| B | 0044794 | biological_process | positive regulation by host of viral process |
| B | 0051014 | biological_process | actin filament severing |
| B | 0051015 | molecular_function | actin filament binding |
| B | 0051293 | biological_process | establishment of spindle localization |
| B | 0061001 | biological_process | regulation of dendritic spine morphogenesis |
| B | 0070062 | cellular_component | extracellular exosome |
| C | 0004672 | molecular_function | protein kinase activity |
| C | 0005524 | molecular_function | ATP binding |
| C | 0006468 | biological_process | protein phosphorylation |
| D | 0000281 | biological_process | mitotic cytokinesis |
| D | 0003779 | molecular_function | actin binding |
| D | 0005515 | molecular_function | protein binding |
| D | 0005615 | cellular_component | extracellular space |
| D | 0005634 | cellular_component | nucleus |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0005856 | cellular_component | cytoskeleton |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0005925 | cellular_component | focal adhesion |
| D | 0007010 | biological_process | cytoskeleton organization |
| D | 0007266 | biological_process | Rho protein signal transduction |
| D | 0009615 | biological_process | response to virus |
| D | 0015629 | cellular_component | actin cytoskeleton |
| D | 0016020 | cellular_component | membrane |
| D | 0016363 | cellular_component | nuclear matrix |
| D | 0022604 | biological_process | regulation of cell morphogenesis |
| D | 0030027 | cellular_component | lamellipodium |
| D | 0030036 | biological_process | actin cytoskeleton organization |
| D | 0030042 | biological_process | actin filament depolymerization |
| D | 0030043 | biological_process | actin filament fragmentation |
| D | 0030424 | cellular_component | axon |
| D | 0030426 | cellular_component | growth cone |
| D | 0031258 | cellular_component | lamellipodium membrane |
| D | 0031982 | cellular_component | vesicle |
| D | 0032587 | cellular_component | ruffle membrane |
| D | 0040019 | biological_process | positive regulation of embryonic development |
| D | 0042995 | cellular_component | cell projection |
| D | 0043066 | biological_process | negative regulation of apoptotic process |
| D | 0044794 | biological_process | positive regulation by host of viral process |
| D | 0051014 | biological_process | actin filament severing |
| D | 0051015 | molecular_function | actin filament binding |
| D | 0051293 | biological_process | establishment of spindle localization |
| D | 0061001 | biological_process | regulation of dendritic spine morphogenesis |
| D | 0070062 | cellular_component | extracellular exosome |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | binding site for residue ANP A 1001 |
| Chain | Residue |
| A | LEU345 |
| A | THR420 |
| A | ASN465 |
| A | LEU467 |
| A | ASP478 |
| A | MG1002 |
| B | SEP3 |
| A | CYS349 |
| A | GLY351 |
| A | VAL366 |
| A | LYS368 |
| A | LEU397 |
| A | THR413 |
| A | GLU414 |
| A | ILE416 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue MG A 1002 |
| Chain | Residue |
| A | HIS464 |
| A | ASN465 |
| A | ANP1001 |
| site_id | AC3 |
| Number of Residues | 14 |
| Details | binding site for residue ANP C 701 |
| Chain | Residue |
| C | LEU345 |
| C | GLY351 |
| C | VAL366 |
| C | LYS368 |
| C | LEU397 |
| C | THR413 |
| C | GLU414 |
| C | ILE416 |
| C | THR420 |
| C | LEU467 |
| C | ASP478 |
| C | MG702 |
| D | ALA2 |
| D | SER3 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | binding site for residue MG C 702 |
| Chain | Residue |
| C | HIS464 |
| C | ASN465 |
| C | ANP701 |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 24 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGKGCFGQAIkVthretgev..........MVMK |
| Chain | Residue | Details |
| A | LEU345-LYS368 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylalanine => ECO:0000269|PubMed:30550596, ECO:0000269|Ref.9, ECO:0007744|PubMed:19369195, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712 |
| Chain | Residue | Details |
| D | ALA2 | |
| C | ASN460 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by NRK => ECO:0000269|PubMed:12837278, ECO:0000269|PubMed:30550596, ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19369195, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| D | SER3 | |
| A | LYS368 | |
| C | LEU345 | |
| C | LYS368 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P18760 |
| Chain | Residue | Details |
| D | SER8 | |
| C | SER337 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| D | LYS13 | |
| D | LYS73 | |
| D | LYS144 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:20068231 |
| Chain | Residue | Details |
| D | THR25 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| D | SER41 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0000269|PubMed:30550596 |
| Chain | Residue | Details |
| D | THR63 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332 |
| Chain | Residue | Details |
| D | TYR68 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:30550596 |
| Chain | Residue | Details |
| D | TYR82 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:30550596 |
| Chain | Residue | Details |
| D | SER108 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455 |
| Chain | Residue | Details |
| D | TYR140 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:30550596, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| D | SER156 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| D | LYS132 |
