5HRH

The crystal structure of AsfvPolX(H115F/R127A mutant): 1nt-gap(P) DNA2:dGTP ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, B)	DNA polymerase beta-like protein	polymer	178	20532.6	2	UniProt (A0A0A1E3N6) Pfam (PF01909) Pfam (PF14791) UniProt (by SIFTS) (P42494)	African swine fever virus (ASFV)	PO174L, PolX
2	B, F (C, F)	DNA (5'-D(*CP*GP*TP*TP*CP*TP*AP*TP*GP*TP*GP*TP*AP*CP*TP*CP*AP*C)-3')	polymer	18	5457.5	2		synthetic construct
3	C, G (D, G)	DNA (5'-D(*GP*TP*GP*AP*GP*TP*AP*CP*A*)-3')	polymer	9	2779.8	2		synthetic construct
4	D, H (E, H)	DNA (5'-D(P*AP*TP*AP*GP*AP*AP*CP*G)-3')	polymer	8	2459.7	2		synthetic construct
5	I, L (A, B)	2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (DGT)
6	J, K, M, N (A, B)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
7	O (A)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 1 - 174 (UniProt: A0A0A1E3N6)

PDB	External Database	Details
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Phe 115	His 115	engineered mutation
Ala 127	Arg 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	62459.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1234.1
All*	Total formula weight	63693.5

*Water molecules are not included.