5HRH
The crystal structure of AsfvPolX(H115F/R127A mutant): 1nt-gap(P) DNA2:dGTP ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 125.500, 70.030, 86.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.986 - 3.000 |
R-factor | 0.2338 |
Rwork | 0.232 |
R-free | 0.27270 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.435 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 15302 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 99.1 | |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% (w/v) PEG 3350, 100 mM HEPES pH 7.5, 200 mM Sodium chloride |