5HR7
X-ray crystal structure of C118A RlmN from Escherichia coli with cross-linked in vitro transcribed tRNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | D, C | tRNA Glu | polymer | 76 | 24378.5 | 2 | GenBank (944398239) | Escherichia coli | |
2 | B, A | Dual-specificity RNA methyltransferase RlmN | polymer | 404 | 45400.8 | 2 | UniProt (A7ZPW0) Pfam (PF21016) Pfam (PF04055) UniProt (by SIFTS) (P36979) In PDB | Escherichia coli | 23S rRNA (adenine(2503)-C(2))-methyltransferase,23S rRNA m2A2503 methyltransferase,Ribosomal RNA large subunit methyltransferase N,tRNA (adenine(37)-C(2))-methyltransferase,tRNA m2A37 methyltransferase |
3 | D, C | MAGNESIUM ION | non-polymer | 24.3 | 7 | Chemie (MG) | |||
4 | B, A | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
5 | B, A | 5'-DEOXYADENOSINE | non-polymer | 251.2 | 2 | Chemie (5AD) | |||
6 | B, A | METHIONINE | non-polymer | 149.2 | 2 | Chemie (MET) | |||
7 | water | water | 18.0 | 94 | Chemie (HOH) |
Sequence modifications
B, A: 1 - 384 (UniProt: A7ZPW0)
PDB | External Database | Details |
---|---|---|
Ala 118 | Cys 118 | conflict |
Gly 385 | - | expression tag |
Asn 386 | - | expression tag |
Ser 387 | - | expression tag |
Ser 388 | - | expression tag |
Ser 389 | - | expression tag |
Val 390 | - | expression tag |
Asp 391 | - | expression tag |
Lys 392 | - | expression tag |
Leu 393 | - | expression tag |
Ala 394 | - | expression tag |
Ala 395 | - | expression tag |
Ala 396 | - | expression tag |
Leu 397 | - | expression tag |
Glu 398 | - | expression tag |
His 399 | - | expression tag |
His 400 | - | expression tag |
His 401 | - | expression tag |
His 402 | - | expression tag |
His 403 | - | expression tag |
His 404 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 139558.6 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 1674.3 | |
All* | Total formula weight | 141232.9 |