5HR7

X-ray crystal structure of C118A RlmN from Escherichia coli with cross-linked in vitro transcribed tRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (D, C)	tRNA Glu	polymer	76	24378.5	2	GenBank (944398239)	Escherichia coli
2	C, D (B, A)	Dual-specificity RNA methyltransferase RlmN	polymer	404	45400.8	2	UniProt (A7ZPW0) Pfam (PF21016) Pfam (PF04055) UniProt (by SIFTS) (P36979)	Escherichia coli	23S rRNA (adenine(2503)-C(2))-methyltransferase,23S rRNA m2A2503 methyltransferase,Ribosomal RNA large subunit methyltransferase N,tRNA (adenine(37)-C(2))-methyltransferase,tRNA m2A37 methyltransferase
3	E, F, G, H, I... (D, C)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
4	L, O (B, A)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
5	M, P (B, A)	5'-DEOXYADENOSINE	non-polymer		251.2	2	Chemie (5AD)
6	N, Q (B, A)	METHIONINE	non-polymer		149.2	2	Chemie (MET)
7	R, S, T, U (D, C, B, A)	water	water		18.0	94	Chemie (HOH)

Sequence modifications

B, A: 1 - 384 (UniProt: A7ZPW0)

PDB	External Database	Details
Ala 118	Cys 118	conflict
Gly 385	-	expression tag
Asn 386	-	expression tag
Ser 387	-	expression tag
Ser 388	-	expression tag
Ser 389	-	expression tag
Val 390	-	expression tag
Asp 391	-	expression tag
Lys 392	-	expression tag
Leu 393	-	expression tag
Ala 394	-	expression tag
Ala 395	-	expression tag
Ala 396	-	expression tag
Leu 397	-	expression tag
Glu 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	139558.6
Non-Polymers*	Number of molecules	13
	Total formula weight	1674.3
All*	Total formula weight	141232.9

*Water molecules are not included.