5HR7

X-ray crystal structure of C118A RlmN from Escherichia coli with cross-linked in vitro transcribed tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	90.717, 70.383, 151.810
Unit cell angles	90.00, 90.11, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.2174
Rwork	0.216
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rfa 2der
RMSD bond length	0.005
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.087	0.529
Number of reflections	75380
<I/σ(I)>	34.9	1.8
Completeness [%]	99.9	99.2
Redundancy	7.3	5.7
CC(1/2)	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	PEG 4000, sodium chloride, sodium cacodylate trihydrate, spermine